HUMAN IMMUNOGLOBULIN BONDING TO GASTRIC CANCER CELL AND CELL STRAIN PRODUCING SAID HUMAN IMMUNOGLOBULIN

摘要

NEW MATERIAL:A human immunoglobulin capable of bonding to gastric cancer cell strain MKN-45 and KATO-III and bonding to the cancer focus of a gastradenocarcinoma section fixed with formalin and embedded in paraffin. USE:Diagnostic and remedy for gastric cancer. PREPARATION:For example, lymph node cells containing human immuno globulin-producing cell are collected from the lymph node around the focus of a human gastric cancer patient, mixed with a B-cell strain ATCC.CRL-8118 and cultured in HAT medium in a carbon dioxide gas incubator to obtain a human immunoglobulin-producing strain as a colony. The target cell strains KATO-III and MKN-45 are separately poured into wells of a microplate and the above collected strain in added to each well to effect the reaction of the strain with the above target strain. The reaction product is made to react with labeled anti-human kappa and lambda (goat) antibody and the cell of the reacted well is further cultured to obtain a human immunoglobulin reactive with the target cell strain.