rekombinantisk, maenniskointerleukin - 2 - synthesis coding plasmid dna of ppr il2 - 19 - type, foerfarande foer otherwise construct of this plasmid dna and maenniskointerleukin - 2 - producer,naemnda plasmid dna innehaallande strain of bacteria culture of escherichia coli vniigenetika vl 903 (ppr il2 - 19)

摘要

A recombinant plasmid DNA pPR-IL2-19, coding for synthesis of human interleukin-2 has a size of 3.85 k.b.p. and consists of the following elements: BamHI-BglII, a fragment of the size of 3.4 k.b.p. of vector plasmid pPR124B obtained on the basis of pPR40 and pML24; Cfr13-BglII, a fragment of the size of 0.45 k.b.p. of plasmid pAA1213-23B obtained by modifying plasmid pAA1213-23 constructed on the basis of plasmid pBR-322 containing the kDNA-copy of the gene of human interleukin-2. A method of construction of the above recombinant plasmid DNA consists in obtaining, by modifying plasmid pAA1213-23, plasmid pAA1213-23B, a fragment of which, together with the gene of interleukin-2, is integrated into vector plasmid pPR-IL2-19, and in transforming, by the thus obtained mixture, cells of E. coli C600 and in separating plasmid pPR-IL2-19 from the clones grown on a selective medium. A strain of bacteria E. coli VNIIGENETIKA VL 903 (pPR-IL2-19) as producer of human interleukin-2 deposited on 12.01.87 under No. 1869 at the culture collection of the USSR Research Institute for Antibiotics.