CLONED DNA CONTAINING TRANSCRIPTION PROMOTING FACTOR S-II, ITS FRAGMENT, PREPARATION THEREOF, PLASMID CONTAINING SAID CLONED DNA OR FRAGMENT AND METHOD FOR DIAGNOSING VIRAL DISEASE AND CANCER USING SAID CLONED DNA OR FRAGMENT

摘要

PURPOSE:To enable mass production of a transcription promoting factor S-II and diagnosis of viral diseases or cancers using the factor with a high accuracy according to a genetic recombination technique, by using a cloned DNA containing the transcription promoting factor S-II and fragments thereof. CONSTITUTION:Cloning of a DNA in a region containing a transcription promoting factor S-II having action of promoting transcription of eucaryotic cells through RNA polymerase was successful for the first time and the structure was determined. It has been found that the amount of expressed mRNA of this transcription promoting factor S-II is 10 times or more based on that of normal cells in cancerous cells or virus-infected cells, especially AIDS virus- infected cells and usable for diagnosis thereof. The formula coding the amino acid sequence of the transcription promoting factor S-II is expressed by the formula.