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PROCESS FOR COUNTING, DETECTING AND IDENTIFYING MYCOPLASMAS IN GENERAL AND UROGENITAL MYCOPLASMAS IN PARTICULAR AND BIOLOGICAL MEDIUM ESPECIALLY ADAPTED TO THIS PURPOSE
PROCESS FOR COUNTING, DETECTING AND IDENTIFYING MYCOPLASMAS IN GENERAL AND UROGENITAL MYCOPLASMAS IN PARTICULAR AND BIOLOGICAL MEDIUM ESPECIALLY ADAPTED TO THIS PURPOSE
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机译:特别适用于此目的的计数,检测和识别特殊和生物介质中的普通和泌尿生殖道小瘤的小瘤
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摘要
In the process described, enzymatic reactions are carried out under anaerobic conditions between a liquid medium for culturing mycoplasmas which serves as a dilution medium for the fluid sample to be analysed and, on the one hand, a first substrate consisting of dehydrated urea or of glucose in the presence of a dehydrated coloured pH indicator, and, on the other hand, a second substrate consisting of dehydrated arginine or of glucose in the presence of a couloured pH indicator. The rate of enzymatic response is determined by noting the time elapsed until the indicator colour change. The respective quantities of urea and arginine or glucose on the one hand and the concentration and the nutrient constituents of the said culture and dilution medium on the other are predetermined and calibrated in such a way that, for Ureaplasma Urealyticum presnet in supra- or infrapathological concentrations the colour change of the indicators is reached or not after a given time-interval.
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