PLASMID HAVING TRANSLATION STARTING- STOPPING-STARTING CODON CONFIGURATION FOR EXPRESSING PROTEIN WITH ESCHERICHIA COLI

摘要

The invention relates to expression plasmids which bring about a considerable increase in expression of proteins, in particular of the thermolabile E.coli enterotoxin subunit B (LT-B). This entails a strong promoter being placed in front of the DNA coding for LT-B so that the so-called Shine-Dalgarno sequence GGGA of the LT-B gene is located six base-pairs (bp) in front of the translation start of the LT-B, and where the stop codon TGA of the LT-A sequence, which precedes in the natural case, is retained.