ANTI-HUMAN UTERUS CANCER ANTIBODY, ITS PRODUCTION AND DETECTION OF HUMAN UTERUS CANCER CELL

摘要

NEW MATERIAL:A monoclonal antibody having specificity against human uterus cancer cell. USE:A diagnostic for uterus cancer. PREPARATION:The objective monoclonal antibody is produced e.g., by immunizing a Balb/c mouse with a human uterus cancer cell, extracting the spleen after the final immunization, collecting antibody-producing cell from the spleen, fusing the cell with a mouse myeloma cell, culturing the fused cell in HAT medium to obtain a hybridoma, selecting a clone capable of producing an antibody having specificity against human uterus cancer cell from the hybridoma, monoclonizing the clone by cloning with limited dilution analysis, etc., and culturing the obtained monoclonal hybridoma.