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FOERFARANDE Foer Reninge AV MAENNISKOLEUKOCYTINTERFERON.

机译:清洗人类学校经皮干扰素的程序。

摘要

A method of rectification of human leukocytic interferon provides for sedimentation of native proteins by means of ammonium sulfate, separation of protein sediment, dissolution of the separated sediment and gel-filtration of the desired product. The sediment is dissolved in a poiser having pH equal to 2.5 to 9.0 and containing a disaggregating agent, whereas before the gel-filtration the column is covered with the same poiser at the amount of 3 to 5 per cent of the volume of the column.
机译:纠正人白细胞干扰素的方法提供了通过硫酸铵沉淀天然蛋白质,分离蛋白质沉淀物,分离的沉淀物溶解和所需产物的凝胶过滤的方法。沉淀物溶解在pH值等于2.5至9.0并含有解聚剂的装瓶器中,而在凝胶过滤之前,用相同的装瓶器覆盖色谱柱,其量为色谱柱体积的3-5%。

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