PROCESS FOR THE PREPARATION OF INACTIVE ADJUVATED BIVALENS VACCINE AGAINST DIARRHOEA OF NEW-BORN LAMBS CAUSED BY ROTA VIRUS AND ESCHERICHIA COLI

摘要

Pilus and virus antigens are used in the prepn. of inactivated, adjuvated, bivalent inoculant against diarrhoea in newborn calves, caused by microorganism namely Rotovirus and Escherichia coli. - The pilus antigen is obtd. by concentrating an inactivated fermented pure culture of E. Coli. gp. of ST+K99 antigen, structure, (No: NCAIMB(P)001098). - Cells are mechanically disintegrated and the antigen is isolated by centrifug ng. - A suspension of a virus-antigen is also prepd. with the use of H.C.S. marked Bovine Rotavirus, (No: NCAIM V(P) 001099). The The Bovine Rotaviruses isolated from newborn calves showing symptoms of diarrhoea is cultured on a monkey-kidney cell line, marked MA-104. The pilus-antigen and the suspension of the virus-antigen are mixed in given proportions, oil contg. adjuvant is added and the prepn. is stored at +4 deg.C. The inoculant is injected into pregnant cows two-or three times. Newly born calves gain immunity by drinking colostrum. - (Dwg.0/0)