METHOD OF STABILIZING HIGH-PURITY FACTOR OF TUMOR NECROSIS FROM RABBIT SERUM OR PLASMA, INDUCED BY LIPOPOLYSACCHARIDES

摘要

PURPOSE:To stabilize the titled factor useful as a carcinostatic agent to keep the activity thereof for a long term even after the separation and purification or freeze-drying step, by adding globulin or protamine to the tumor necrotic factor (TNF). CONSTITUTION:A tumor necrotic factor (hereinafter abbreviated to TNF) is stabilized by adding globulin, e.g. bovin blood serumal globulin, or protamine, e.g. protamine of a salmon, or a salt thereof thereto. For using the TNF induced in an animal or a tissual culture system as a carcinostatic agent, the separation and purification are important, and the storage in a solution or a frozen state for a long term is essential. However, the activity is remarkably reduced by the operation. The addition of the globulin and/or protamine inhibits the reduction in the activity. The concentration of the added globulin and/or protamine is 1mug or more, optimally 100mug or more, based on 1ml solution containing the TNF. Preferably, the separation and purification are carried out at 0-30 deg.C, and the freeze-drying is carried out at 0 deg.C or below.