ACCELERATION OF GROWTH OF RECOMBINANT HOST CONTAINING MUTAGEN IN BIOSYNTHESIS OF CELL WALL AND/OR CELL MEMBRANE AND ACCELERATION ACCELERATION OF EXPRESSION OF MUTATIONAL PROTEIN IN SAID RECOMBINANT HOST

摘要

PURPOSE: To efficiently produce a heterologous protein by culturing S. cerevisiae having an expression cassette in a medium containing each proper amount of assimilative nitrogen source, an amino acid, saccharide and microelement(s). ;CONSTITUTION: First, a medium for growing recombinant cells for expressing extraneous protein is prepared by including an assimilative nitrogen source, an amino acid, saccharide and microelement(s) and regulating the respective molar concentrations thereof so as to represent penetration degrees optimum for host cell growth. In the above medium, the aimed heterologous protein is produced by inducing its production in mnn9 yeast cells containing an expression cassette suitable for such production and bearing at least one variance in the biosynthesis of cell wall or plasma membrane constituents. The above- mentioned penetration degree is suitably 533-1,050 mOsm.;COPYRIGHT: (C)1991,JPO