PREPARATION OF CHITINASE BY WASABIA JAPONICA-CULTURED CELL, PREPARATION OF CHITINASE/LYSOZYME, PURIFICATION OF CHITINASE, PURIFICATION OF CHITINASE/LYSOZYME

摘要

PURPOSE:To readily prepare the subject new enzyme also having a strong lysozyme activity in high purity by culturing the tissue pieces or cell pieces of the genus Wasabia plant in an artificial nutritive medium. CONSTITUTION:The tissue pieces or cell pieces of the genus Wasabia plant is cultured in an artificial nutritive medium to prepare the objective enzyme. The enzyme is purified by allowing chitin powder to adsorb a cultured filtrate obtained from the cultured product.