SELECTIVE ADSORBENT FOR HUMAN ALPHA2-PLASMIN INHIBITOR AND SEPARATION OF HUMAN ALPHA2-PLASMIN INHIBITOR IN HUMAN PLASMA

摘要

PURPOSE:To separate alpha2-plasmin inhibitor in blood plasma, by using a selective adsorbent for human alpha2-plasmin inhibitor prepared by bonding a specific monoclonal antibody to an insoluble carrier. CONSTITUTION:A selective adsorbent for human alpha2-plasmin inhibitor is produced by bonding an insoluble carrier (e.g. sepharose,polyacrylamide, cellulose, etc.) with a monoclonal antibody (Japanese Patent Application, April 17, 1984) capable of specifically recognizing and bonding the site inhibiting the fibrinolytic activity of plasmin in human alpha2-plasmin inhibitor. The alpha2-plasmin inhibitor in plasma can be separated by the affinity chromatography using said adsorbent. A patient of alpha2-plasmin inhibitor deficient disease is liable to cause grave hemorrhage by the premature disintegration of the thrombus.