RECOMBINANT PLASMID DNA PIF, ENCODING POLYPEPTIDE WITH PROPERTIES OF HUMAN LEUKOCYTE INTERFERON @2, AND A STRAIN OF BACTERIA ESCHERICHIA COLI - A PRODUCER OF POLYPERIPTIDE WITH PROPERTIES OF HUMAN LEUKOCYTE INTERFERON @2

摘要

the invention u043eu0442u043du043eu0441u0438u0442u0441u00a0 biotechnology, including genetic engineering, and can be used in medicine. purpose u0438u0437u043eu0431u0440u0435u0442u0435u043du0438u00a0 u00a0u0432u043bu00a0u0435u0442u0441u00a0 increased u0443u0440u043eu0432u043du00a0 synthesis of interferon. received u043fu043bu0430u0437 - meade pIF 14, u043au043eu0434u0438u0440u0443u044eu0449u0430u00a0 u043au043eu043du0441u0442u0438u0442u0443u0442u0438u0432u043du044bu0439 fusion u043fu043eu043bu0438u043fu0435u043fu0442u0438u0434u0430 with properties of u043bu0435u0439u043au043eu0446u0438u0442u0430u0440u043du043eu0433u043e interferon.u0440u0435u043au043eu043cu0431u0438u043du0430u043du0442u043du0430u00a0 u043fu043bu0430u0437u043cu0438u0434u0430 pIF 14 contains a tandem synthetic genes, interferon, u044du043au0441u043fu0440u0435u0441u0441u0438u00a0 which occurs by u0442u0440u0430u043du0441u043bu00a0u0446u0438u0438 is u043bu0438u0446u0438u0441u0442u0440u043eu043du043du043eu0439 matrix rna, to u043eu0442u043eu0440u043eu0439 u0442u0435u0440u043cu0438u043du0438u0440u0443u044eu0449u0438u0439 codon preceding the u0446u0438u0441u0442u0440u043eu043du0430 tao part of the sequence u00a0u0432u043bu00a0u0435u0442u0441u00a0 shyne - u0434u0430u043bu044bu0430u0440u043du043e TAAGGA in the initiation u0442u0440u0430u043du0441u043bu00a0u0446u0438u0438 distal u0446u0438u0441u0442 ron.u044du043au0441u043fu0440u0435u0441u0441u0438u00a0 tandem genes, interferon u043au043eu043du0442u0440u043eu043bu0438u0440u0443u0435u0442u0441u00a0 combination u043au043eu043du0441u0442u0438u0442u0443u0442u0438u0432u043du044bu0445 u043fu0440u043eu043cu043eu0442u043eu0440u043eu0432 a2 and a3 early area of bacteriophage t7.u0440u0435u043au043eu043cu0431u0438u043du0430u043du0442u043du0430u00a0 u043fu043bu0430u0437u043cu0438u0434u043du0430u00a0 dna pIF 14 consists of Hindlll / Kpnl - fragment dna plasmids containing tandem pTNF311 u043fu0440u043eu043cu043eu0442u043eu0440u043eu0432 a2 and a3 in the early field of bacteriophage t7 gene / lac - t u0430u043cu0430u0437u044b and transcription terminator u0444u0430u0433u0430 i, and Kpnl / Hindlll - fragments containing synthetic site initiation u0442u0440u0430u043du0441u043bu00a0u0446u0438u0438 and tandem two induced genes u043bu0435u0439u043au043eu0446u0438u0442u0430u0440u043du043e the human interferon.obtained strain of escherichia coli, which provides a high level of biosynthesis u043bu0435u0439u043au043eu0446u0438u0442u0430u0440u043du043eu0433u043e interferon and 2 people (more than 35% of the total cellular protein) in u0443u043fu0440u043eu0449 u0435u043du043du043eu0439 technologies u0432u044bu0434u0435u043bu0435u043du0438u00a0. 2 boeing. s - lu. (l to x | on o yu