STRAIN OF HYBRID CULTURED MAMMALIAN CELLS MUS MUSCULUS L - A PRODUCER OF MONOCLONAL ANTIBODY, INTERACTING WITH @@@-SUBUNITЪ OF HUMAN CHORIONIC GONADOTROPIC HORMONE AND HUMAN LUTEINIZING HORMONE

摘要

the invention u043eu0442u043du043eu0441u0438u0442u0441u00a0 to medicine and biotechnology.the use of u043fu0440u043eu0434u0443u0446u0438u0440u0443u0435u043cu044bu0445 strains no. u0432u0441u043au043a (p) 397u0434 u043cu043eu043du043eu043au043bu043e of antibodies (u043cu043eu043du0430u0442) u043fu043eu0437u0432u043eu043bu00a0u0435u0442 develop immunological methods u043eu043fu0440u0435u0434u0435u043bu0435u043du0438u00a0 u0443u0440u043eu0432u043du00a0 u0445u043eu0440u0438u043eu043du0438 and guna - u0434u043eu0442u0440u043eu043fu0438u043du0430 rights (hcg) in biological u0436u0438u0434u043au043eu0441u0442u00a0u0445 organism u0434u043bu00a0 diagnosis of pregnancy among women at an early date, diag - u043du043eu0441u0442u0438u043au0438 u0445u043eu0440u0438u043eu043du044du043fu0438u0442u0435u043bu0438u043eu043c s and other u0442u0440u043eu0444u043eu0431u043bu0430u0441u0442u0438u0447u0435u0441u043au0438u0445 tumors, peak u043eu0432u0443u043bu00a0u0446u0438u0438 women.the strain obtained by hybridization cells of mice u043cu0438u0435u043bu043eu043cu043du043eu0439 line x 63 ad 8.653 cells from spleen of mouse BALB / c, u0438u043cu043cu0443u043du0438u0437u0438u0440u043eu0432u0430u043du043du043eu0439 stated the u043du0430u043bu044cu043du044bu043c drug chorion u0438u0447u0435u0441u043au043eu0433u043e guna - u0434u043eu0442u0440u043eu043fu0438u043du0430 rights (hcg). | 3 as u0441u043bu0438u0432u0430u044eu0449u0435u0433u043e agent used peg 1500. u0441u0435u043bu0435u043au0446u0438u00a0 cells held at; environment HAT, implemented u043au043bu043eu043du0438u0440u043eu0432u0430u043du0438u00a0 method 2 limit u0440u0430u0437u0432u0435u0434u0435u043du0438u0439, received 100% positive clones.u0443u0441u043bu043eu0432u0438u00a0 u0432u044bu0440u0430u0449u0438u0432u0430u043du0438u00a0 strain: u0441u0440u0435u0434u0430.u044fu0440u043c - 1640u0441 10% bovine serum u0444u0435u0442u0430u043bu044cu043du043eu0439 temperature 37 & deg; c, u0433u0430u0437u043eu0432u0430u00a0 phase air with 5% u0441.u043e2. - way u043au0443u043bu044cu0442u0438u0432u0438u0440 u043eu0432u0430u043du0438u00a0 in plastic bottles of 50 to 250 ml, u043fu043eu0441u0435u0432u043du0430u00a0 dose of 500000 cells / ml, 1 shot screening: 2, u0432u0440u0435u043cu00a0 u0441u0443u0431u043au0443u043bu044cu0442u0438u0432u0438u0440u043eu0432u0430u043du0438u00a0 3 - 4 u0434u043du00a0.in 10 to 20 days after the u0438u043du043eu043au0443u043bu00a0u0446u0438u0438 cells strain in abdominal cavity of mice BALB / c treated u043fu0440u0438u0441u0442u0430u043du0435u043c receive u0430u0441u0446u0438u0442u0438u0447u0435u0441u043au0443u044e fluid. with the help of u0432u044bu0441u0430u043bu0438u0432u0430u043du0438u00a0 u0441u0435u0440u043du043eu043au0438u0441u043bu044bu043c ammonia and subsequent u0438u043eu043du043eu043eu0431u043cu0435u043du043du043eu0439 chromatography on a column with u0434u0435u0430u0435 - 52 u0446u0435u043bu043bu044eu043bu043eu0437u043eu0439 from 1 ml of au - u0446u0438u0442u0438u0447u0435u0441u043au043eu0439 fluid u0432u044bu0434u0435u043bu00a0u044eu0442 employment about 7 mg immunoglobulin content. u043cu043eu043du0430u0442. u043fu0440u043eu0434u0443u0446u0438u0440u0443u0435u043cu044bu0445 strain, up to 90%.u043cu043eu043du0430u0442 react, hcg and u043bu0433u0447 in breeding 1 mg / ml in u0438u043cu043cu0443u043du043eu0444u0435u0440u043cu0435u043du0442u043du043eu043c and ria methods. the strain is deposited with the u0432u0441u043au043a (p) under no. 397u0434. q (4 n3 o o