TOXOPLASMA GONDII SPECIFIC GENE PROBES, AND THEIR USE FOR IN VITRO DETECTION OF TOXOPLASMA GONDII AND FOR THE TYPING OF TOXOPLASMIC STRAINS.

摘要

The screening of toxoplasmosis is described, wherein a rapid, highly sensitive and routinely applicable detection of Toxoplasma gondii is sought. For this purpose, nucleic acids forming all or part of DNA sequences selected from TGR1A, TGR1E, TGR2 and TGR4, or the corresponding complementary sequences or said sequences modified by substitutions and/or additions and/or the deletion of one or more nucleotides, are used in such a way as to leave their properties unchanged. According to the method for detecting Toxoplasma gondii, specific oligonucleotides are selected, the DNA to be analyzed and contained in a biological sample is amplified by a polymerase chain reaction method, the amplified DNA fragments are separated, denatured, transferred and fixed to a membrane, and hybridization is attempted using one of the selected oligonucleotides, which is labelled. Said nucleic acids can also be used for typing Toxoplasma gondii strains.