METHOD OF PRODUCING EXTRACELLULAR ENZYMES, THE ENZYMES PRODUCED THEREBY AND COMPOSITIONS CONTAINING THEM; A HYBRID PLASMID AND A DNA FRAGMENT COMPRISING DNA ENCODING THE ENZYMES; A MICROORGANISM HARBOURING THE PLASMID; AND A METHOD FOR REMOVING NUCLEIC ACIDS FROM BIOLOGICAL MATERIALS

摘要

Extracellular Serratia spp. enzymes have been found to be excreted by another gram-negative organism harbouring a plasmid carrying DNA from Serratia spp. encoding the enzymes. This organism, eg. E. coli, is therefore employed to produce the enzymes, specific examples of Serratia spp. enzymes produced are a nuclease, a lipase and a phospholipase. The nuclease may be employed to remove nucleic acids from a biological material.