Preparation of esterase genes, esterases, toxin plasmids and transforming microorganisms, and optically active carboxylic acids and their colon esters using the transforming microorganisms

摘要

The present invention is represented by general formula (I);The amino acid sequence of esterase which sub-hydrolyzes the carboxylic acid ester represented by (wherein R ₁ represents an alkyl group, an aralkyl group or an aryl group, R ₂ and R ₃ represents an alkyl group, n represents 1 or 2). DNA fragments comprising a base sequence encoding, esterases encoded in the DNA fragments, a switch plasmid containing the DNA fragments, a transformed microorganism by the switch plasmid, and a transformed microorganism. It relates to a method for producing activated carbon acid and its counterpart esters.