STRAIN OF HYBRIDOUS CULTURED MAMMALIAN CELLS - MUS MUSCULUS L - A PRODUCER OF PROTECTIVE MONOCLONAL ANTIBODIES TO EQUINE EAST ENCEPHALITIS VIRUS

摘要

. Use: biotechnology, immunology. SUMMARY OF THE INVENTION: Hybrid continuous cell strain was obtained by fusion of mouse myeloma cells P3NS1 / 1-Ag4.1 syngennyh mice with BALB / c cells immunized with the purified virus preparation, Southern embodiment. A hybridoma secreting monoclonal antibody lgG2, specifically interact with the viral glycoprotein E2 VsEL neutralizing virus infectivity on Vero cells te molecules with a titer of 1: 200 and protecting sensitive animals from leta--negative virus doses administered at passive. Strain designated 1B2, and stored under the number (S) 479D, antibody titer reached 1: 5000 in the culture fluid and 1: 300,000 in the ascites. Table 1. 1 yl. ate with