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A novel system of inducible positive regulation of use for production of heterologous proteins
A novel system of inducible positive regulation of use for production of heterologous proteins
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机译:一种新型的诱导性正调控系统,用于生产异源蛋白
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摘要
The description relates to a novel system of inducible positive regulation of use in the construction of vectors reproducible by expression in host microorganisms for controlled production of heterologous proteins in high yields. More particularly the system comprises the vir EcoRI-XhoI region of about 4.5 kb contained in the chromosomal DNA of Bordetella which codes for one or more regulating factors, and a terminal 5′ region situated in front of the promotor of the chromosomal Bordetella genes capable of being activated or repressed in trans by the aforementioned factors.;The description also relates to vectors which can be replicated by expression and contain the regulating system and microorganisms transformed by the vectors.
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机译:该描述涉及诱导性正向调控系统的新用途,该系统用于构建可通过在宿主微生物中表达而可再现的载体,以高产量控制生产异源蛋白。更特别地,该系统包括 Bordetella U>的染色体DNA中包含的约4.5 kb的 vir U> EcoRI-XhoI区域,其编码一个或更多的调控因子,以及位于能够被上述因子反式激活或抑制的染色体 Bordetella U>基因启动子前的5'末端。还涉及可通过表达复制的载体,其包含调节系统和由载体转化的微生物。
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