Isolated microspore and anther culture of corn

摘要

This invention relates to the regeneration of fertile monocotyledonous plants from anther or microspore cultures. Fertile corn plants have been regenerated from in vitro cultures by the methods disclosed herein. The invention relates to synergistic effects of pretreatment with a combination of stresses, for example, a carbon structure in the preculture medium capable of inducing embryogenesis such as mannitol at cold temperatures, for example, 10° C. A solid support system has been developed to transfer isolated microspores through a series of subcultures containing different media. The composition of the media are aspects of the invention. Chromosome doubling methods for haploid anther and isolated microspore cultures are presented. This invention also relates to the plants regenerated from microspore or anther cultures, and to their seeds and progeny.