The revelation secretion vector null which possesses DNA and the aforementioned DNA array which include the field where it participates in the revelation and the secretion of the protein

摘要

PURPOSE:To provide Escherichia coli, etc., with extracellular production ability, by preparing DNA containing a promoter range participating in manifestation of gene of alkaline bacterium of the genus Bacillus, a liposome range and a protein secretion participating range. CONSTITUTION:DNA containing a promoter range participating in manifestation of gene of starch hydrolyzing enzyme of alkali bacterium of the genus Bacillus, a liposome range and a range participating in extracellular secretion of Escherichia coli or bacterium of the genus Bacillus of protein produced as the result of manifestation. DNA sequence derived from cyclomaltodextrin glucanotransferae of alkali bacterium of the genus Bacillus is preferable as the DNA sequence containing a range participating in secretion of protein produced as the result of manifestation of gene.