QUANTITATIVE ANALYSIS OF LACTOPROTEIN CONTENT IN FOOD BY USING ELISA, MONOCLONAL ANTIBODY SELECTED FOR THE ANALYSIS AND SELECTION OF THE MONOCLONAL ANTIBODY

摘要

PURPOSE: To obtain a monoclonal antibody capable of quantitatively analyzing lactoprotein in a food even after denaturation or decomposition by using a partial peptide of β-lactoglobulin as an antigen and selecting the antibody under a condition where it is combined with the antigen, the original protein and the reducing carboxymethylated substance. ;CONSTITUTION: A partial peptide of β-lactoglobulin represented by formula I to VI is used as an antigen and combined with KLH of a carrier protein by using a binder such as glutalaldebyde to prepare a peptide-KLH conjugate material. This conjugate material is administrated together with an adjuvant to a BALB/c mouse to immunize the mouse therewith. After the final immunization, splenic cells are collected and a mouse myeloma cell is fused therewith. The fused cell is cultured in an HAT selection medium to obtain a hybridoma. Selection of the resultant hybridoma is then carried out under a condition where all the antigen peptide, β-lactoglobulin and the reducing carboxymethylated β-lactoglobulin can be combined and the positive clone is cultured, thus affording the objective new monoclonal antibody capable of quantitatively analyzing lactoprotein in a food without fail even when it is affected by thermal degeneration and gegradation.;COPYRIGHT: (C)1995,JPO