the genes coding for the tripsine of anopheles gambiae and the regions of dna that control the expression in the intestine.

摘要

in a library developed in the fago? embl3a, using dna from anopheles gambiae (suakoko xag karyotype, 2r + 2, '+ 37 +) were isolated two partially overlapping dna inserts (ty4.1 and ty3.3), see drawing. ty3.3 consists of 14748 nucleotides and contains seven genes (t1, t2, t3, t4, t5, t6, 17), which encode for many proteins.the analysis of the sequence of amino acids of proteins encoded by these genes indicates the presence of extensive similarities with several enzymes of the family of serino protease.the transcription of t1, t2, t3, t4 and 17 is induced at the level of the intestine exclusively by taking blood from the mosquito.the recombinant proteins, encoded by t1, t2, t3, t4 and 17.expressed in escherichia coli possess a selective proteasica activity towards different components of serum and red blood cell.these results indicate that t1, t2, t3, t4 and 17 encode for the tripsine of a. gambiae involved in the process of digestion of the blood meal.the possible applications of sequences t1, t2, t3, t4, t5, t6 and 17 and sequences of ty3 intergeniche.3 concern: 1) the genetic control of vectors of disease through the development of transgenic mosquitoes that produce pesticides and / or antiviral agents in the intestinal lumen.2) the control of mosquitoes by the immunization of individuals and animals with tripsine, 3) development, biochemical and biological methods.the invention relates to: (1) the use of dna sequences that line the genes of tripsine as components of vectors for the expression of heterologous sequences in transgenic mosquitoes or ecllule bug, 2) the use of sequences of amino acids of t1, t2, t3, t4, t5, t6 and 17 or parts of them to develop antibodies to the carrier, and 3) the use of sequences of amino acids of t1, t2.t3, t4 t5, t6 and 17 in all the biochemical processes and biological in vitro, which require proteolytic activity.