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METHOD OF SELECTION OF COLD RESISTANT CORN GENOTYPES

机译:耐冷玉米基因型的选择方法

摘要

FIELD: agriculture. SUBSTANCE: selection is carried out by pollen collection, effect with 0.005 M benzidine solution in acetate buffer, pH 4.7. Mixture is kept for 5-10 min, 0.01% solution of hydrogen peroxide is added, and resistance to cold of genotypes is determined by color change intensity for 20-60 s. Colorless is typical for cold resistant genotypes (0-1 point), light blue and blue for mean resistant genotypes (2-3 points) and dark blue for nonresistant to cold genotypes (4-6 points). EFFECT: improved method of corn selection. 1 tbl, 1 dwg
机译:领域:农业。物质:通过花粉收集进行选择,用0.005 M联苯胺溶液在pH 4.7的乙酸盐缓冲液中起作用。将混合物保持5-10分钟,加入0.01%的过氧化氢溶液,并通过20-60s的颜色变化强度来确定基因型的耐寒性。无色是典型的耐寒基因型(0-1分),浅蓝色和蓝色代表平均抗性基因型(2-3分),深蓝色代表非耐寒基因型(4-6分)。效果:改良的玉米选择方法。 1桶,1桶

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