Process of enriching adherent CD4.sub.+ T cells from monocyte depleted peripheral blood mononuclear cells with interleukin 2 and interleukin 4

摘要

Peripheral blood mononuclear cells (PBMC) are treated to deplete monocytes and the remaining cells are cultured in a medium containing interleukin-2 (IL-2) and interleukin-4 (IL-4). Nonadherent cells are removed and discarded, and adherent cells are further cultured in a medium of IL-2 and IL-4 which expands the CD4+ T cells population of the PBMC. The expanded, enriched CD4+T cells can be placed in a pharmaceutically acceptable carrier and administered to a mammal with IL- 2 to treat a tumor.