In eukaryotic cells, and retroviral vectors for transfer and expression of genes for therapeutic purposes

摘要

(57) A retroviral vector recombinant for transferring and / or cloning and / or expression of exogenous nucleotide sequences, [Abstract] The present invention is between 1527 and nucleotide number 7702 in the sequence shown in Figure 1 consists of any of the sequences contained in the ClaI-PvuII fragment which is substantially located, LTR sequence contained between 144 and nucleotide number 7842, PBS site beginning at nucleotide number 145, the sequence of 250 nucleotides following the end of the LTR sequences and comprises a packaging sequence included in, the direction of transcription is also a transfer in either direction with respect to the direction of transcription of the viral, said sequence, to be able to control the transfer and / or expression and cloning of the exogenous sequences and / or on retroviral vector recombinant characterized. The present invention relates to the use of the vector for the cloning and / or expression and / or therapeutic purposes, and in particular the introduction of the gene.