A collection of phagemids, a collection of Escherichia coli cells carrying the phagemids, a collection of phagemid particles produced from said collection and phagemid particles obtained according to the process

摘要

The invention concerns a collection of phagemids comprising a promotor, a gene of a fusion between a ligand protein, wherein the ligand protein in its processed state would comprise of from 40 to 60 amino acid residues, and a bacteriophage protein, at least one transcription terminator sequence, a replication origin derived from a single strand bacteriophage, a plasmid replication origin and facultatively at least one selection marker. Further, the invention concerns a collection of Escherichia coli clones or cells representing the said collection of phagemids, a process for isolating phagemid particles with strong binding characteristics for a defined target molecule with the use of collections according to the invention and phagemid particles obtained according to the process.