Vitamin B-mediated oral delivery system for GCSF and EPO (VITAMIN B MEDIATED ORAL DELIVERY SYSTEMS FOR GCSF AND EPO)

摘要

The present invention relates to a method of preparing a complex VB₁₂ RTI ID = 0.0 (IF) /RTI in the GCSF or EPO portion of the complex₁₂Analogs and complexes of GCSF or EPO. The present invention also relates to a method of synthesizing these complexes. This is VB₁₂ RTI ID = 0.0 GCSF /RTI or EPO. Preferably the complex has the formula V-X-A-Y-Z wherein V is vitamin B₁₂Or vitamin B₁₂/RTI ₁₂Or via a central cobalt atom to X, or via VB₁₂And X is-NHNH-,-NH-,-O-,-S-,-SS-, or-CH₂-, A is an optionally substituted, saturated or unsaturated, branched or straight chain C RTI ID = 0.0 _One-₅₀Wherein Y is a covalent bond between A and Z and is selected from the group consisting of-NHCO-,-CONH-,-CONH-,-CONH-, ,-CONHNHCO-,-N = N-,-N = CH-,-NHCH₂-,-NHN = CH-,-NHNHCH₂-,-SS-,-SCH₂-,-CH₂S-,-NHCRNH-,-COO-,-OCO-, R is selected from O, S or NH₂And Z is GCSF or EPO. The present invention also describes a reagent which can be used as a probe for the detection of a thiol group contained in a protein or peptide, wherein the reagent has a terminal reactive group capable of forming a disulfide bond with a free thiol in the protein or peptide Vitamin B RTI ID = 0.0 ₁₂(Or an analogue thereof) or a complex of any detectable label, either mechanically or visually