Method for producing taxane type diterpene and method for obtaining taxane type diterpene-producing cultured cell

摘要

The present invention relates to a method for producing a taxane-type diterpene comprising culturing the tissue or cells of a plant producing the taxane type diterpen using at least one selected from the group consisting of jasmine essential oils, heavy metal-containing compounds, complex ions containing heavy metals, heavy metal ions, amines, Wherein the oxygen concentration in the gas phase in the incubator is controlled from the initial stage of culture under conditions less than the oxygen concentration in the atmosphere in culturing the tissue or cells of the plant, Or a culture medium having a fluidity in contact with a tissue or a cell under the condition that the dissolved oxygen concentration in the medium is lower than the saturation dissolved oxygen concentration at that temperature and is cultured under control from the beginning of the culture. A method for producing a taxane-type diterpene, which comprises culturing the plant tissue or cells by using a gas containing 0.03 to 10% carbon dioxide gas as an oxygen-containing gas to be introduced into the tank, The method of claim 1, wherein the cells of the plant are divided into a plurality of layers depending on the difference in specific gravity, and the cells contained in at least one layer are cultured to select the taxane type diterpene-And to a method for obtaining high-yield cultured cells. According to the present invention, it is possible to industrially produce a taxane-type diterpene such as taxol, which is useful as a cancer treatment agent.