Novel L-sorbose dehydrogenase and novel L-sorboson dehydrogenase obtained from Gluconobacter oxydans T-100

摘要

The present invention encodes novel L-sorbose dehydrogenase (SDH) and novel L-sorbosone dehydrogenase (SNDH), SHD and / or SNDH derived from Gluconobacter oxidans T-100. SDH and / or SNDH characterized by culturing the DNA, an expression vector comprising the DNA, host cells transformed with the expression vector, and host cells in the medium and recovering SDH and / or SNDH from the obtained culture. It relates to a manufacturing method of. SDHs and SNDHs of the invention are useful enzymes with desirable properties for the preparation of 2-geto-L-gulonic acid and L-ascorbic acid. According to the preparation method of the present invention, SDH and SNDH having such desirable properties can be produced by Darang by genetic engineering.