RECOMBINANT PLASMID DNA ECO 1831RI ENCODING SYNTHESIS OF RESTRICTION ENDONUCLEASE ECO 1831RI, STRAIN OF BACTERIUM ESCHERICHIA COLI - A PRODUCER OF RESTRICTION ENDONUCLEASE ECO 1831RI

摘要

FIELD: genetic engineering. SUBSTANCE: new recombinant plasmid pECO 1831RI (size is 8.1 thousand nucleotide pairs) has genes of restriction-modification system Eco 1831RI and consists of Bam HI-fragment of vector pUC19 and BglII-fragment of the natural plasmid p1831, three recognition sites by restrictase Eco RI, two sites - StyI, and one site for restrictases: KpnI, SalI, HindIII, PstI, SmaI, and resistance determinant to ampicillin also. Obtained plasmid provides increased synthesis of restrictase Eco 1831RI. Proposed strain E. coli BKM CR - 3680D prepared by transformation with plasmid DNA pECO 1831RI into the strain E. coli R802 provides the high level of enzyme content, 2500000 U/1 g wet bimass, not less. The yield of purified enzyme is 350000 U/g biomass. EFFECT: increased yield of enzyme. 3 cl