Isolation of DNA from biological prepn(s). and amplification of highly degraded DNA

摘要

Process for isolating DNA from biological prepns. comprises digesting proteins by incubation with a 1-2.5 mg/ml protease soln. for several hours, incubating the resulting soln. again in a closed vessel under the same conditions, removing the digested proteins, precipitating the remaining DNA fragments under mild conditions, and washing the pptd. DNA with alcohol to remove contaminants. Also claimed is a process for amplifying highly degraded DNA, comprising amplifying CA repeats from the variable number of tandem repeats (VNTR) regions of the DNA using primers selected so that only fragments with a size of 150 bp or less are amplified.