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Solution SELEX and light selection of nucleic acid ligands: systematic evolution of ligands by exponential enrichment
Solution SELEX and light selection of nucleic acid ligands: systematic evolution of ligands by exponential enrichment
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机译:解决方案SELEX和核酸配体的光选择:通过指数富集系统地进化配体
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摘要
A method for identifying nucleic acid ligands to target molecules using the SELEX procedure wherein the candidate nucleic acids contain photoreactive groups and nucleic acid ligands identified thereby are claimed. The complexes of increased affinity nucleic acids and target molecules formed in the procedure are crosslinked by irradiation to facilitate separation from unbound nucleic acids. In other methods partitioning of high and low affinity nucleic acids is facilitated by primer extension steps as shown in the figure in which chain termination nucleotides, digestion resistant nucleotides or nucleotides that allow retention of the cDNA product on an affinity matrix are differentially incorporated into the cDNA products of either the high or low affinity nucleic acids and the cDNA products are treated accordingly to amplification, enzymatic or chemical digestion or by contact with an affinity matrix.
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