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MUTANASE-PRODUCING MICROORGANISM AND MUTANASE
MUTANASE-PRODUCING MICROORGANISM AND MUTANASE
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机译:产生诱变酶的微生物和诱变酶
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摘要
PURPOSE: To obtain a new mutanase having excellent suppressing effect on the formation of dental plaque and sufficiently stably miscible to form a product of a composition for oral cavity application. ;CONSTITUTION: Bacillus sp. RM1 strain (FERM P-14836) is cultured and the objective mutanase is collected from the culture product. The mutanase has high activity to decompose the α-1,3-glucoside bond of mutan and optimum working condition of pH4 and 60°C, stably keeps its activity at pH4-10 and ≤60°C and has a molecular weight of about 150,000 by SDS-polyacrylamide gel electrophoresis. The activity of the enzyme is inhibited by mercury, silver, trivalent iron and p-chloromercury benzoic acid. A synergistic plaque-removing effect can be achieved by the combined use of the enzyme with dextranase.;COPYRIGHT: (C)1996,JPO
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