METHOD FOR OPTIMIZATION OF BINDING PROTEINS AND NUCLEIC ACIDS ENCODING A BINDING PROTEIN PRODUCED THEREBY

摘要

A method for manipulating nucleic acids so as to optimize the binding characteristic of an encoded binding protein, comprising: (a) providing two or more nucleic acids encoding functionally related binding proteins having at least one set of splicing sites, said set of splicing sites flanking opposite ends of one or more encoded binding domains; (b) mixing said two or more nucleic acids to produce a parent population of mixed nucleic acids encoding functionally related binding proteins; and (c) randomly incorporating said binding domains between said two or more nucleic acids through said set of splicing sites to produce a different population of nucleic acids encoding binding proteins wherein at least one binding protein is characterised by substantially different binding characteristics than a member of the parent population.