METHODS OF IDENTIFYING INDUCIBLE INHIBITORS OF TRANSFORMATION PROGRESSION.

摘要

This invention provides a method of identifying a progression suppressor gene which comprises: a) introducing DNA containing a progression gene into a population of transformed cells; b) treating the introduced transformed cells from step (a) so as to induce the expression of at least one progression suppressor gene and cause a subpopulation of the cells to revert to a characteristic phenotype; c) selecting cells which express the progression suppressor gene and exhibit the characteristic phenotype; d) isolating mRNA from the cells from step (c); e) comparing the mRNA so obtained with mRNA obtained from the uninduced transformed cells as to identify mRNA expressed only by the cells from step (c); and f) isolating the gene encoding such mRNA so as to thereby identify the progression suppressor gene.