Procedure for the detection and quantification of diarrheic toxins of dinoflagellates (DSP (diarrhoetic shellfish poison) based on the inhibition of phosphatases.

摘要

PROCEDURE FOR TOXINS detection and quantitation of Diarrheal dinoflagellates (DSP (DIARRHOETIC SHELLFISH POISON)) based on the inhibition of phosphatases. THE PRESENCE OF RED TIDE PRODUCING DSP CONTAMINA mollusks, which must be continuously monitored before marketing. The current technique ES bioassay, which involves administering a EXCERPT mice, the result is positive if the mouse DIES BEFORE 12 HOURS. This method is slow, expensive, erratic and unspecific. PROCEDURE POISON detected and quantified by measuring inhibition partially purified phosphatases. Inhibiting phosphatases SUPPORT IS MEASURED using as a microtiter plate, and revealing enzyme activity with a substrate of phosphatases according to its characteristics CHEMICAL BECOMING A fluorescent product, LUMINESCENT OR COLOR. THIS technique is fast, safe and sound. THE SECTOR WILL BE FAVORED THE PRODUCTION INDUSTRY YCONSERVERA MOLLUSC.