Japanese encephalitis-pandemic hemorrhagic fever vaccine

摘要

The present invention relates to a Japanese encephalitis- pandemic hemorrhagic fever mixed vaccine, which is used in 1-7 days old mice or primary hamster kidney epithelial cells with immature brain cells instead of 3 to 4 week old mice, which are currently used during the manufacturing process of Japanese encephalitis vaccine. Japanese encephalitis virus was cultured to prepare a vaccine. As a result, the contents of brain protein and Myelin basic protein of the newly improved Japanese encephalitis vaccine were significantly reduced to 1 / 10-1 / 100 of the previous vaccine. In addition, prophylactic vaccines were prepared by culturing 84/105 and 76/118 strains of the Hantan virus causing epidemic hemorrhagic fever. Meanwhile, the Japanese encephalitis- pandemic hemorrhage mixed vaccine was prepared by mixing the amount of Japanese encephalitis virus antigen prepared by the above method to 20-80 units / 0.1 ml, and the amount of Hantan virus antigen to 1.024 unit / 0.1 ml or more. The mixed vaccine of the present invention prepared as described above has no side effects that occur in the infiltration of brain proteins, and one preventive vaccine can simultaneously provide a preventive effect against two diseases.