Production method of regeneration ginseng plant by somatic embryo culture

摘要

In this invention, a spherical embryo grown from somatic embryos harvested from cotyledons extracted from a phosphate seed is inoculated into a solid culture incubator to which dioxins and cytokinins are added to a MS medium with a nitrogen concentration, Growth process The cotyledon breed developed was inoculated to MS medium supplemented with modified dioxin and cytokinin on nitrogen-modified MS medium, cultured at 4-25 ° C for 2-6 weeks, Culturing the cotyledon breeder at a temperature of 4-25 ° C in a solid culture medium supplemented with gibberellin in an MS medium having a nitrogen concentration differentiated to germinate the ginseng plant. According to the present invention, a spherical embryo which has propagated somatic embryos of ginseng is developed into a cotyledon, and then subjected to a sufficient maturation process, so that a ginseng plant having stem and root is the same as the parent plant is obtained.