DNA sequences encoding enzymes that can facilitate the synthesis of linear -1, 4 glucans in plants, fungi and microorganisms (DNA SEQUENCES CODING FOR ENZYMES CAPABLE OF FACILITATING THE SYNTHESIS OF LINEAR -1,4 GLUCANS IN PLANTS, FUNGI AND MICROORGANISMS)

摘要

The present invention provides a DNA sequence encoding a protein having the enzymatic activity of amylose sucrose synthesizing linear -1, 4 glucan from sucrose, by bacteria, fungi and plants or in a cell-glass system; Plasmids and bacteria containing the DNA sequences; A method for producing a plant and a microorganism capable of expressing a polypeptide having an amylose sucrase activity intracellularly or extracellularly; And a protein exhibiting an enzymatic activity of amylose sucrose to produce pure fructose, wherein the DNA sequence is prepared by the following steps.;(a) preparing a genomic or cDNA library based on genomic DNA or mRNA of an organism cell; (b) transforming the appropriate host with the library prepared according to (a); (c) adding iodine vapor to the transformed cells; (d) identifying cells stained with blue color; (e) isolating and culturing the cells identified in (d); (f) isolating genomic DNA inserts or cDNA inserts from the transformed cells.