Random the cDNA/genome the bacteriophage/huajimido expression library which includes the DNA which is cut off or cloning gene

摘要

(57) Abstract Being the method of identifying the peptide 1 or more it possesses living thing unique affinity vis-a-vis specified structure, the said method, in order (a) the cloning gene, DNA fragment of cDNA or genome DNA origin to fuse with the gene of the surface protein of the helper bacteriophage, making connect, the stage which draws up bacteriophage/huajimidoraiburari, and (b) includes the stage which selects the bacteriophage particle which is connected to specified structure. At the same time said bacteriophage/huajimidoraiburari is different kind vis-a-vis the bacteriophage and the cloning gene, it possesses the individual member who possesses the gene of the surface protein which is fused to the DNA fragment of cDNA or genome DNA origin, the peptide which the cord/code is done is disclosed the case of fusion with the surface protein on the surface of the individual bacteriophage particle member by said fragment.