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RECOMBINANT TYPE HUMAN HEPATOMA CELL-DERIVED GROWTH FACTOR AND ANTIBODY CAPABLE OF RECOGNIZING HUMAN HEPATOMA CELL-DERIVED GROWTH CANCER
RECOMBINANT TYPE HUMAN HEPATOMA CELL-DERIVED GROWTH FACTOR AND ANTIBODY CAPABLE OF RECOGNIZING HUMAN HEPATOMA CELL-DERIVED GROWTH CANCER
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机译:重组型人肝细胞源性生长因子和可识别人肝细胞源性生长因子的抗体
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摘要
PROBLEM TO BE SOLVED: To obtain a procaryotic expression vector containing a gene capable of encoding a human hepatoma-derived growth factor(HDGF), a procaryotic cell transformed with the vector, a recombinant type human HDGF, prepared from the transformed procaryotic cell and having growth activities and an antibody capable of recognizing the human HDGF. ;SOLUTION: This recombinant type human hepatoma cell-derived growth factor protein undergoes the procaryotic expression and has growth activities. The protein is expressed by a procaryotic host cell containing an expression vector containing a DNA sequence capable of encoding the growth factor protein. Furthermore, the protein is a procaryotic expression vector containing the DNA sequence capable of encoding the human hepatoma cell-derived growth factor protein operably connected to a procaryotic promoter sequence. The antibody is capable of recognizing the human hepatoma-derived growth factor and reactive with a human hepatoma-derived growth factor prepared by purifying a culture supernatant of a human hepatoma-derived cultured cell HuH-7.;COPYRIGHT: (C)1999,JPO
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