Formation manner null of tissue culture seedling

摘要

PROBLEM TO BE SOLVED: To provide a method for forming the tissue culture seedling of a stock, capable of obtaining the normal tissue culture seedling of the stock in a high efficiency by effectively suppressing the formation of a water immersed seedling or a water immersed plant body in culturing the callus induced from the piece of a tissue of the stock or the callus obtained by maintaining and growing the callus. ;SOLUTION: This method for forming a tissue culture seedling of a stock consists of the first culturing process obtaining small lumps of seedlings or plant bodies by culturing the calluses induced from the pieces of the tissue of the stock or the calluses obtained by maintaining and growing the calluses in a high concentration culturing medium containing 1.0-4.0wt.% culture medium solidifying agent and the second culturing process obtaining the tissue culture seedlings of the stock by culturing the small lumps of seedlings or plant bodies obtained in the first culturing process in a low concentration medium containing the culture medium solidifying agent within the range of 0.1-1.4wt.% and lower than that used in the first culturing process.;COPYRIGHT: (C)1997,JPO