Obtaining highly pure eicosapentaenoic acid from microalgae phaedactylum tricornutum by a three step process.

摘要

THE eicosapentaenoic acid (EPA) HIGH INTEREST TO HUMAN HEALTH, has been obtained from the microalgae Phaeodactylum PIX through a process of three stages: EXTRACTION OF FATTY ACIDS BY DIRECT saponification BIOMASS HUMEDA; CONCENTRATION Polyunsaturated fatty acids (PUFAs) BY THE METHOD OF UREA AND PURIFICATION EPA liquid chromatography (HPLC). EXTRACTION OF FATTY ACIDS HAS BEEN PERFORMED WITH KOH-ethanol (96% V / V) (1H, 60 C) extracted 90.9% EPA present in the biomass. UREA / FATTY relationship has been 4: 1 P / P, the crystallization temperature 28 C HAS BEEN USED methanol solvent UREA, giving a yield of 78.6% EPA. EPA purification was performed on a column of 4.7 cm ID X 30 cm reverse phase C18 using methanol-water (1% ACH) 80:20 P: P AS MOBILE PHASE; 97.3% EPA CHARGED IN COLUMN HAS RECOVERED with a purity of 95.8%. The overall yield of the process is 69.5%. EPA PRODUCTION OF HIGH PURITY OF 2.46 G / DIA, DETERMINED BY BIOMASS PRODUCTION Phaeodactylum PIX A photobioreactor tubular outer (100 g / day). This same process is applicable to other MICROALGAE AND OTHER PRODUCING PUFAs.