Method for the identification of target genes for transcription factors

摘要

Identifying transcription factor responsive elements and/or target genes and/or cofactors of transcription factors comprises: (a) introducing a gene trap vector into a eukaryotic cell in culture, where the vector contains a reporter gene, a polyadenylation sequence and a selectable marker gene; (b) selecting cells containing the vector; (c) contacting the cells with a transcription factor; (d) identifying and culturing cells that exhibit a change in reporter gene activity; and (e) identifying the target genes and/or cofactors of the transcription factors and/or the transcription factor responsive elements.