Simultaneous Differential Diagnosis of Nephrotic Bleeding Fever, Tsutsugamus Disease, Leptospirosis, Immunoblotting and Diagnostic Kit

摘要

The present invention relates to a simultaneous differential diagnosis method of nephrotic bleeding fever, Tsutsugamus disease, leptospirosis using immunoblot, and more particularly, to blot the antigens of nephrotic bleeding fever, Tsutsugamus disease and leptospirosis at a predetermined position on the nitrocellulose membrane, respectively. Renal bleeding hemorrhagic fever, comprising: reacting the sample and the control serum with the nitrocellulose membrane to which the antigens are blotted, detecting the reactive antibody and comparing the detection result in the sample and the control serum, , Simultaneous differential diagnosis of leptospirosis.;The present invention also relates to a diagnostic kit comprising an antigen-blotting nitrocellulose membrane obtained by blotting antigens of nephrotic bleeding fever, Tsutsugamus disease and leptospirosis, respectively, at a predetermined position on the nitrocellulose membrane, and a method for preparing the same.;According to the present invention, it is possible to easily diagnose nephrotic bleeding fever, Tsutsuma's disease, and leptospirosis at the same time without a skilled inspector and special equipment.