METHOD OF PREPARING HCV PROTEINS USEFUL FOR USING IN VACCINE OR IMMUNE ANALYSIS, ASIALOGLYCOPROTEIN (VARIANTS), COMPOSITION FOR USING IN VACCINE OR IN IMMUNE ANALYSIS (VARIANTS), METHOD OF ASIALOGLYCOPROTEIN PURIFICATION AND A METHOD OF HCV CONTENT DECREASE OR ELIMINATION

摘要

FIELD: molecular biology, virology. SUBSTANCE: method of HCV proteins preparing involves host cell culturing that is transformed with structural gene encoding protein HCV followed by expression of structural gene and isolation of protein HCV from cell culture. Obtained protein is an asialoglycoprotein taken from E1, E2 or their aggregates. Protein is isolated by cell culture contact with mannose- -binding protein followed by separation of cell culture part interacting with protein and binding mannose. Invention can be used for diagnosis, treatment and prophylaxis of hepatitis C virus infection. EFFECT: improved method of preparing and purification of protein. 26 cl, 4 ex