EXPRESSION EUCARYOTIC VECTOR 'P CAT-3' FOR CLONING FOREIGN DNA FRAGMENTS AND INVESTIGATION OF SIGNALS OF POSTTRANSCRIPTION CONTROL OF GENE EXPRESSION CONTAINING IN FRAGMENTS

摘要

FIELD: molecular biology, genetic engineering. SUBSTANCE: invention relates to an eucaryotic expression vector ensuring to clone different DNA fragment by a single stage. Invention proposes a vector "p CAT-3" constructed on the base of the known plasmid "p CAT-Control". Vector has a bacterial gene encoding enzyme chloramphenicol acetyl transferase (CAT) as a gene-reporter. In this plasmid a region Bpi I/Hpa I locating lower a gene CAT is substituted with a site consisting of two synthetic oligonucleotide adaptors and sites of polylinkers of plasmid "p Blueskript//SK". Indicated site has 14 unique sites for usual restriction endonucleases and ensures to clone easily different DNA fragment in it. EFFECT: higher efficiency. 4 dwg, 1 tbl, 1 ex