Non-hematopoietic cells, including cardiomyocytes and skeletal muscle cells, derived from hematopoietic stem cells and methods of making and using them

摘要

The present invention provides a process of promoting differentiation of a stem cell into a cardiomyocyte or skeletal muscle cell, comprising the steps of obtaining a stem cell, which is preferably a hematopoietic stem cell, with cardiomyocyte or skeletal muscle cell potential from a donor and contacting the stem cell with a growth factor or combination of growth factors. The invention also provides a population of cardiomyocytes or skeletal muscle cells derived using the process and the nonembryonic stem cells having cardiomyocyte or skeletal muscle cell potential or embryonic or nonembryonic hematopoietic stem cells. Further provided is a composition, comprising the stem cells and a combination of growth factors in amounts and conditions to promote the differentiation of the stem cells into cardiomyocytes or skeletal muscle cells. Also provided are methods of using the cells of the present invention.