Chemotherapy Treatment for HIV-positive Patients Based on Phenotypic Drug Sensitivity of Human HIV Strain

摘要

HIV chemotherapy treatment for HIV positive patients transfects cell lines susceptible to HIV with sequences from the pol gene of HIV obtained from the patient and HIV-DNA constructs lacking those sequences, Cultivating the transfected cells to produce chimeric virus stocks, evaluating and assigning a value to the phenotypic susceptibility of said chimeric virus to inhibitors of said enzymes encoded by the pol gene of HIV , Constructing a data set consisting of the above values for chimeric viral susceptibility and corresponding values for chimeric wild type strains of HIV, and repeating sensitivity assessments for at least two or more other inhibitors in total to repeat at least three such data sets The difference between the chimera and wild-type susceptibility for each data set. Represent the data set described above in the form of two- or three-dimensional graphs in a manner that provides a visual measure of the resistance of chimeric stocks for treatment with the inhibitors in question and based on the graphical representations of the measured resistances thus determined. And selecting the optimal inhibitor. This method produces large-scale, economical and rapid phenotypic information about individual HIV-infected patients. This method is applicable to all currently available chemotherapy regimens and is expected to apply consistently to future chemotherapy regimens.